Comparison of Ultrasonic Extraction with Conventional Extraction Methods of Phenolic Compounds in Longan(Euphoria longana Lamk.) Seed.
Three different extraction methods - hot water (HWE), ethanol (EE) and ultrasonic-assisted water extraction (UAE) - were evaluated for extraction of phenolic compounds in longan (cultivar Edor) seed. The findings indicated that longan seed extracts contained three major phenolic compounds, analyzed by HPLC, including gallic acid, corilagin and ellagic acid. The content of total polyphenolic compounds and antioxidant activities was determined using the Folin-Ciocalteu method and the DPPH assay, respectively. Longan seed extraction by HWE contained the highest yield, total polyphenol content and antioxidant activities (42.80%, 41.250mg gallic acid/g and IC50 = 0.017 mg/ ml, respectively). UAE produced a higher yield than EE, but lower than HWE, with the lowest extraction time. Moreover, gallic acid content (16.55 mg/g) and corilagin content (35.62 mg/g) extracted by UAE were higher than those of HWE and EE. Ellagic acid content obtained from UAE was significantly (P<0.05) higher than that from EE, but not significantly different from that of HWE. The application of UAE could provide an alternative method for extraction of photochemical from longan seeds.